Objective: This study was designed to lead to an understanding of the factors influencing patient satisfaction in pain clinic at a medical center in southern Taiwan. Methods: The data was collected from July 1 to December 1, 2013 via a 12- item questionnaire that included 6 treatment-related and 6 environment-related items. Patients in a pain clinic who needed to receive a nerve block were randomly selected and asked to fill out the questionnaire. A statistical analysis with a multiple ordinal regression was used to describe and examine the factors that influence patient satisfaction. Results: A total of 96 patients completed this study. Among the 12 items in the questionnaire, staff follow-up, nurse attitude, and staff patience were among the highest 3 percentiles in the very strongly agree category [64/96(66.7%), 63/96(65.6%), 62/96(64.6%), respectively], which are all treatment-related satisfaction indices. On the other hand, waiting time and traffic were among the lowest 2 percentiles in the very strongly agree category [31/96(32.3%) and 41/96(42.7%), respectively], which are both environment-related satisfaction. Overall treatment-related satisfaction was higher than environment-related satisfaction. Visit frequency was the only significant predictor of treatment-related satisfaction. Meanwhile, marital status and visit frequency were significantly associated with environment-related satisfaction. In the multiple ordinal regression analysis, less frequent visits and poorer self-assessed health status were associated with lower satisfaction in both treatment-related satisfaction and environment-related satisfaction. Conclusion: This study showed that patients were generally satisfied with the pain clinic in terms of both treatment and environment and that treatment-related satisfaction was higher than environment-related satisfaction. We found that patient satisfaction was associated with visit frequency and self-assessed health status and that environmental factors should also be considered to improve quality of health care.